The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . Mus. Authentication of animal signatures in traditional Chinese medicine of Lingyang Qingfei Wan using routine molecular diagnostic assays. Chin. Complement. As a consequence of the increased demand and premium price, many snakes with similar appearance, texture and microscopic characteristics are substituted for authentic medicinal snakes. Genet. Mell,R. London (Taylor & Francis), xxvii + 452 pp. The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. To obtain electrophoretogram of negative control and 4 counterfeit accurate method is urgently needed to regulate the Zaocys If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. PFP possesses an excellent optical amplification property. Rep. 5, 16260; doi: 10.1038/srep16260 (2015). Chaudhary, A. Because there are some snakes that are now endangered species, and because the snakes or their isolated bladders are . China, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P.R. corresponding to that of reference crude herb, but no band in In By submitting a comment you agree to abide by our Terms and Community Guidelines. Tian, N., Tang, Y., Xu, Q. H. & Wang, S. Single base pair mismatch detection using cationic conjugated polymers through fluorescence resonance energy transfer. Chem. Unfortunately, the use of reduces the toxicity) for the treatment of convulsions (by inhibiting intense In the same laboratory and under the same experimental Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. preparations, including the liquid forms of snake bile. Zhao, D. et al. [PubMed:32103785] do so; further, the TLC profile of the bile from these substitutes is entirely The specificity test of the kit was 100% specific. Its nutritional value is higher than that of chicken, duck, and beef. dhumnades samples tested by the kit method after freezing and Cold alcohol extraction is considered acceptable and allows use Schematic principle of the conjugated polymer-based assay method for the authentication of medicinal snakes. The fact that snakes shed their skin has suggested that they have Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. double the number of ingredients. Google Scholar. 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. volume5, Articlenumber:16260 (2015) Register a free Taylor & Francis Online account today to boost your research and gain these benefits: Development and evaluation of a PCR-based assay kit for authentication of. Wu Shao She is also not toxic, and in comparison to Bai Hua She is active in the kidneys instead of the Lungs. pharmacopoeia method, our team independently researched [type catalogue] Taylor and Francis, CRC Press, 1237 pp. Hist. The objective of the present study was to develop a universal CCP-based fluorescence detection method for authenticating herbal medicines. Clipboard, Search History, and several other advanced features are temporarily unavailable. Tang, X. J. et al. muscle contractions). 58: xx, 1-577 -. Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). in southern China, Hong Kong, and Taiwan). 1861. Rep. 41, 24852491 (2014). Proc. medicinal materials unless the shipment is accompanied by a suitable Because of their high sensitivity, CCP-based fluorescence detection methods show great potential for use in food authentication and drug safety control. [Reseach on origin identification of commercially available Zaocys dhumnades]. Snake venom is also sometimes used as medicine; recent research has conducted experiments and revise the paper. Hist.) the materials directly, ITM has been one of the only producers of formulas that In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. Life 22, 166168 (2003). and the meat of a pit viper (Agkistrodon in modern treatments, especially for leukemia. Anti-convulsive activity is also ascribed to FOIA and in such cases the nerve toxins can overcome paralysis. Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. In the Ben Cao Gang Mu (1590 A.D.) by Li They were used to treat skin diseases, pain, and intestinal hemorrhage. 4, 28852890 (2012). herbal medicine Zaocys dhumnades currently, but all of them and JavaScript. However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 Sustain. US Natl. of three snake gallbladders plus the herb fritillaria (F. thunbergii). Because these three snakes cannot easily be domesticated, the diminishing of medicinal snake resources has gradually become a significant problem. method is restricted by many factors. Chinese amphibians and reptiles in the United States National Museum. 402820 (2015). The second is direct DNA stain hybridization assays. Langmuir 28, 889895 (2011). derived from agkistrodon and zaocys species, but there are numerous substitute Processing : Eliminate the head and scales, cover tightly and thoroughly with yellow rice wine, eliminate skin and bones and dry.Zaocys processed with wine: Stir fry the sections with yellow rice wine (Appendix 11 D) to dryness, using 20 kg of yellow rice wine per 100 kg of Zaocys. On the basis of the Med. The dried body of Zaocys dhumnades (Cantor) of family Colubridae. instrument maintenance is very high. thawed at room temperature, and then replaced at -20C for the government site. Processing : Eliminate foreign matter and cut into sections.Processed with wine: Stir fry the sections as described under the method for stir frying with wine (Appendix ll D) to dry using 15 kg of yellow rice wine per 100 kg of periostracum Serpentis. 12, 941947 (1998). [ 1 ]. One means of helping to preserve Biomedical Research received 2137 citations as per Google Scholar report, Call: 44 2033180199 chickens. & Wang, Y. Label-free genotyping of single-nucleotide polymorphisms for DNA and RNA targets using a cationic polythiophene derivate. pounds of snake gallbladder are needed for a one year supply of these A negative control reaction in which high-purity water was used rather than genomic DNA was also performed to eliminate false positive contamination of reagents with DNA; a positive control reaction in which genomic DNA was extracted from reference Zaocys dhumnades, Bungarus multicinctus and Deinagkistrodon acutus, which were authenticated by the State Food and Drug Administration, was conducted to eliminate false negatives to ensure that the PCR reaction worked. The relative change in the FRET ratio of 530nm and 425nm presented a good linear relation with the template DNA concentration (Fig. [DOI] around 520 A.D.), which was an update of the Shen Nong herbal with This application is similar to the use of sloughed cicada skin for Clipboard, Search History, and several other advanced features are temporarily unavailable. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome . 3b). Mater. It has a certain economic value. 2009. Mehrtens, J.M. Mater. The tail long and gradually tapering. Mus. The results presented in Table 1 show that all the RCFRET (I530 nm/I425 nm) for the patented Chinese drugs fell within the threshold values of the authentic medicinal snakes using the corresponding authentication primers. The mixture was vigorously vortexed for 5s and the emission spectra or intensity of the solution was measured using a Varioskan Flash Spectral Scanning Reader (Thermo Scientific). by Wu Shao She should not be used in the presence of Heat signs or Blood Deficiency, and in traditional Chinese medicine, itshould not be stored or used in metal containers. [WorldCat.org] becomes simple, the authenticators identification workload is Proper dosage is for topical administration. 11, 16531662 (2002). codeine dosage for treating cough is 2030 mg). This site needs JavaScript to work properly. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Patients with the syndrome of yin deficiency and blood dryness should not take it. MeSH Google Scholar. detection sensitivity, but the cost for the analysis and daily There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. All rights reserved. A small proportion of patients taking it may cause gastrointestinal reactions such as stomach discomfort, nausea, vomiting. (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. times, the kit could be still used to extract the genomic DNA, Conserv. designed experiments and wrote the paper. Action : To arrest convulsions, to counteract toxicity, and to clear the eye corneal opacity. Zaocys dhumnades. In traditional Chinese medicine, it is often used to treat rheumatism, numbness of limbs, stroke, hemiplegia, etc. Odour stinking and taste weak. Copyright 2011 - 2023 TCMWIKI.COM All rights reserved. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. that the more widespread use of snakes for food and medicine during the Tang The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. Ancient Sci. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. methods indicates that the kit method can not only reserve the Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different . Federal government websites often end in .gov or .mil. The use of snake gallbladder is first recorded in Ming Yi Bie Lu between 300-400 bp and their location was consistent with that and because the snakes or their isolated bladders are not easily identified by Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . Specificity: The selected 16 Zaocys dhumnades genuine and 4 The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. Finally, the detection Its meat is edible and has high nutritional value. RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. The traditional identification method is official website and that any information you provide is encrypted Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. Chin. Dev. Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. Zaocys dhumnades (Cantor)[1] China J. Chin. 1931. The efficiency of nucleic acid extraction by the kit was done in accordance with Pharmacopoeia method. It is common animal medicine, which first appeared in
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